Journal: Cancer Science

Article Title: Achaete‐scute family bHLH transcription factor 2 activation promotes hepatoblastoma progression

doi: 10.1111/cas.16051

Figure Lengend Snippet: Sphere‐formation assay in the induced expression experiment with HepG2 Tet‐On ASCL2. (A) Representative images from the sphere‐formation assay using the HepG2 Tet‐On ASCL2 cells from the Dox (−) and Dox (+) treatment groups on day 7. Sphere sizes were larger with the Dox (+) treatment than with the Dox (−) treatment. Scale bar = 0.5 mm. (B) Sphere‐formation abilities of the HepG2 Tet‐On ASCL2 cells were evaluated on day 7 using the sphere‐formation assay. There were more spheres with the Dox (+) treatment than with the Dox (−) treatment (mean [SD] 515.0 [63.1] spheres vs. 412.8 [44.4] spheres, P = 0.038, n = 4). Day 7: culture period after seeding in an Asnol Petri dish. Values shown are the means ± SD. * P < 0.05. Dox, doxycycline.

Article Snippet: For the induced expression experiment, HepG2 Tet‐On ASCL2 cells (5 × 10 4 ) were cultured with or without Dox after seeding on φ90 × 20 mm Asnol Petri dishes (AS ONE Corporation) for 7 days (Figure ).

Techniques: Tube Formation Assay, Expressing